New release 2019/06/04
Tissue engineering technology and Rapid Prototype Technology
Material information for test
Sol-gel material
Type H ( High temperature type )
Material name Poly N – isopropylacrylamide / ( PIPAAm )
Linear Formula C6H11NO
LCST 63 degree Celsius
CAS No. 2210-25-5
Molecule wt 113.16
CAT No. 07089-02 / Packaging 25g/3000JPYEN
NACALAI Co
Type M – 1 ( Middle temperature type )
Material name : Poly N – isopropylacrylamide / ( PIPAAm )
Linear Formula (C6H11NO)n
LCST 32 degree Celsius.
CAS No. 25189-55-3
MDL number MFCD00284282
Molecule wt average Mn 20,000-25,000
CAT No. 535311-10G / Packaging 10g in glass btl / 1136.87Rm / 1380Rm
SIGMA-ALDRICH
Type M – 2 ( Middle temperature type )
Material name : Poly N – isopropylacrylamide / ( PIPAAm )
Linear Formula (C6H11NO)n
LCST 30 degree Celsius.
CAS No.
MDL number
Molecule wt average Mn
Production name D-MEM/F-12
CAT No. 12400024 / Packaging 10g in glass btl / 11500 JPYEN
Invitrogen
Type L ( Low temperature type )
Material name : Poly (N-isoproplyacrylamide) – b – Poly (Ethylene Glycol ) 5g LOT. No 2P0186
Molecular formula C2n+2H4n+6On+2
CAS No. 25322-68-3
This is changeable of LCST from 8 to 40 degree Celsius. We use normal y LCST by 22 degree Celsius.
A) Cell
NIH 3T3 mouse fibroblasts ( cell line ) / Primary respiratory epifhelial cells
Invitrogen Corporation GIBCO
Dulbeccos Modified Eagle Medium : D-MEM/F-12 ( CAT No.12400-024 LOT No.400681 )
Lung A549
NCI-H292 + interferon ( IFN )
B) Cellulose oxidized mesh ( fiber mesh or cotton ) for hemostasis use
based alpha-cellulose
When used for oral applications this product typically resolves 7 – 14 days.
Solve in 10% weight medium of cloth weight
Production name Surgicel
Maker Johnson & Johnson ( Ethicon ) / Sorbacel ( Germany ) / Traumacel ( Bioster,Czech ) / Alltracel ( Ireland )
1)Collagen
Own fat or Hyaluronic acid or Poyacrylamide gelCollagen One
: Skin, tendon, vascular, ligature, organs, bone / Collagen Two
: Cartilage / Collagen Three
: Reticulate Collagen Four
: Cell
BAXTER AG Vienna Austria TISSEEL Kit Powders and Solvents for Sealant Kit No.VNT1H022
2) Alginatce powder
Calcium Alginate is transport from Na and Ca and sometimes not clear.
3) Pluronic
Pluronic F127 is almost same spec than PIPAAm. LCST temperature is same 4 degree Celsius
Step 1 30% ( wt/vol) F-127 : Culture media
Step 2 Mix slowly in cold room
Step 3 120 degree Celsius 20min autoclaved
Step 4 Stock in cold place from 0 to 4 degree Celsius.
Ethylene Oxide / Propylene Oxide Block Copolymer
Name Pluronic F127 Prill Surfactant Product 583106
Maker BASF Corporation Mount Olive New Jersey 07828-1234
4) PLGA
This material is useful for bone scaffold generation. But chemical reaction is about 170 degree Celsius and we need to another combine material.
5) Fibrin ( Commercial / patients plasma )
6)Cadherin-17
Culture Dish for culture
Up side
:30mm dish ( Nunclon Surface CAT. No 153066 / LOT. No 085528 )
Down side
:30mm Glass bottom dish (0.17mm bottom) (WillCo-Dish Ref. NoGWST3522 / LOT. No 0803-137 )
For solve
:50ml( CELLSTAR CAT. 227261 / LOT.No07400115 )
For test plus
:TC-PLATE 6 WELL, STERILE WITH LID, SINGLE PACKED CAT. No 657 160 / LOT. No 07260151
For plan
:1536 well high base plate 10.4mm SBS/ANSI ( Nunc CAT. No 164707 )
Culture medium
FD + DMM + amino acid + glucosa + Na+ + K+ + H2O + NaHCO3 + Antibroties + (VitC+Gluslaminc acid+)
IGF-1 ( Insulin like growth factors )
PDGF + IGF-1
Enzymes ( Collagenases )
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